Electroporation of phaeodactylum tricornutum

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August undefined, 2024

WebApr 3, 2024 · The promoter/terminator of the nitrate reductase gene of the model diatom Phaeodactylum tricornutum was cloned and used to drive chloramphenicol acetyltransferase (CAT) reporter gene expression. The construct was transferred by electroporation into P. tricornutum grown in medium lacking silicon. CAT expression … WebAug 27, 2024 · Phaeodactylum tricornutum (P. tricornutum) is a marine diatom 5–27 μm in length and 3–4 μm in width belonging to the Phaeodactylaceae family. P. tricornutum can be found in different morphotypes (i.e., fusiform, triradiate and oval) that may have different behaviors under different downstream processing conditions.

Electroporation Transformation Protocol for …

WebApr 1, 2024 · In this study, the correlation between the specific growth rate of Phaeodactylum tricornutum and fucoxanthin (fucoxanthin) biosynthesis was investigated both in batch and in semi-continuous cultures. Fucoxanthin content from P. tricornutum biomass showed a positive correlation with specific growth rate during the … WebSuccessful transformation of Phaeodactylum tricornutum (CMP632) by electroporation was achieved based on a modified version of Zhang & Hu (2014). palm bay urgent care fl

High-efficiency nuclear transformation of the diatom …

WebAug 20, 2024 · Phaeodactylum tricornutum is a model diatom with defined culture conditions, but routine genetic manipulations are hindered by a lack of simple and robust genetic tools. ... In contrast to ... WebApr 23, 2013 · A highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that drives multi-sequence pulses to introduce foreign DNAs into the cells. WebAn efficient nuclear transformation method has been established for the pennate marine diatom Phaeodactylum tricornutum using an electroporation system that drives multisequence pulses to introduce foreign DNAs into the cells. By removing excess salts in the culture medium and optimizing pulse condi … palm bay view north cyprus

Transformation of diatom Phaeodactylum tricornutum by …

Laminaria japonica hydrolysate promotes fucoxanthin accumulation …

WebThe diatom P. tricornutum, shown in figure 1, was purchased from the CCAP algae collection and grown in purified domestic seawater mixed with Guillard’s f/2 medium … WebSuccessful transformation of Phaeodactylum tricornutum (CMP632) by electroporation was achieved based on a modified version of Zhang & Hu (2014). … sun bum car air freshener amazonWebJan 1, 2024 · Fucoxanthin (Fx) has gained a growing attention due to the remarkable biological activities. The limited biomass of was the restrictive factor for Fx production in Phaeodactylum tricornutum.In this study, Laminaria japonica hydrolysate (LPH) with a low addition proportion of 1.5 ml/L, was proved to promote fucoxanthin accumulation and cell … palm bay weather underground

"WebJan 11, 2024 · Episome-Based Gene Expression Modulation Platform in the Model Diatom Phaeodactylum tricornutum (Journal Article) DOE PAGES DOE PAGES ® Journal Article: Episome-Based Gene Expression Modulation Platform in the Model Diatom Phaeodactylum tricornutum Episome-Based Gene Expression Modulation Platform in … " - Electroporation of phaeodactylum tricornutum

Electroporation of phaeodactylum tricornutum

Electroporation Transformation Protocol for …

WebCurrently, training through the Biochemistry PhD program in Dr. Karas's lab, which focuses on developing innovative strategies for genome engineering. I study Phaeodactylum tricornutum, a eukaryotic micro-algae, and have created a platform for rapid organelle genome engineering in this model system. My research interests include: 1) organelle … WebApr 23, 2013 · A highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that …

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WebApr 8, 2024 · Lhcx proteins provide photoprotection via thermal dissipation of absorbed light in the diatom Phaeodactylum tricornutum. 13 September 2024 ... The electroporation parameters were set as follows ... WebJun 1, 2012 · In a study using microalgae Phaeodactylum tricornutum, the pHY18 vector was ligated with gene of interest DGAT2 and then transformed by electroporation …

WebJan 1, 2024 · In this study, the marine diatom Phaeodactylum tricornutum was selected as the model organism since it presents many advantages, including culture easiness and … WebApr 3, 2024 · The promoter/terminator of the nitrate reductase gene of the model diatom Phaeodactylum tricornutum was cloned and used to drive chloramphenicol acetyltransferase (CAT) reporter gene expression. The construct was transferred by … Pdf/Epub - Transformation of diatom Phaeodactylum tricornutum by … BioTechniques is a MEDLINE-indexed, peer-reviewed, open access journal that …

WebThe diatom P. tricornutum, shown in figure 1, was purchased from the CCAP algae collection and grown in purified domestic seawater mixed with Guillard’s f/2 medium recipe (Guillard, 1975), rather standard in cultivation of marine diatoms, ingredients are shown in table 1. Figure 1. Phaeodactylum tricornutum Bohlin (1897). CCAP 1055/15. WebJul 15, 2024 · Phaeodactylum tricornutum is a marine pennate species that has become an experimental model used to understand diatom biology. The nuclear genome is chimeric, containing genes derived from ancestral endosymbioses with red and green algae, and enriched by genes acquired laterally from bacteria.

WebA highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that drives multi …

WebAug 27, 2024 · The efficiency of PEF-assisted extraction combined with aqueous or dimethyl sulfoxide (DMSO) solvents in recovering pigments and polyphenols from microalgae Tetraselmis chuii (T. chuii) and Phaeodactylum tricornutum (P. tricornutum) was evaluated. Two PEF treatments were applied: (1 kV/cm/400 pulses, 3 kV/cm/45 pulses), … palm bay trash scheduleWeb宋燕子贾彬林柏成胡章立黄瑛（深圳市海洋生物资源与生态环境重点实验室深圳市海洋藻类开发与应用工程实验室深圳大学生命科学学院，深圳 518060） palm bay wedding venuesWebPhaeodactylum was the first diatom to be successfully genetically engineered ( Zaslavskaia et al., 2001) and the genome has also been sequenced and annotated ( Bowler et al., … palm bay well drillersWebHighly efficient transformation of the diatom Phaeodactylum tricornutum by multi-pulse electroporation Epub 2013 Apr 7. Authors Mado Miyahara 1 , Masaki Aoi , Natsuko Inoue-Kashino , Yasuhiro Kashino , Kentaro Ifuku Affiliation 1 Graduate School of Biostudies, Kyoto University, Kyoto, Japan. PMID: 23563551 DOI: 10.1271/bbb.120936 … palm bay wastewater treatment plantWebMay 26, 2009 · Phaeodactylum tricornutum is the most promising diatom exploited to date, especially as a platform of pharmaceutical production. Herein, we expose diatoms’ main features that allowed using them ... palm bay tree service palm bay flWebKey words: diatom; electroporation; multi-pulse; Phaeodactylum tricornutum Diatoms are important primary producers in the ecosystem that have crucial roles in the global carbon … sun bum car freshener sun bum car air freshener