Human monocytic cell line
WebKeywords. Blast Crisis. Mononuclear Phagocyte System. Monocytic Cell Line. Naphthyl Acetate. Diffuse Histiocytic Lymphoma. These keywords were added by machine and not … U-937 cells are a pro-monocytic model cell line used in biomedical research. They were isolated from the histiocytic lymphoma of a 37-year-old male patient in 1974. Due to the relative uniformity of expanded cultures and lower maintenance requirements these cells have been since used as an important tool for studying phagocyte differentiation and different kinds of cell-to-cell interactions. U-937 cells mature and differentiate in response to a number of soluble stimuli, adopting the morp…
Human monocytic cell line
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Although THP-1 cells are of the same lineage, mutations can cause differences as the progeny proliferates. In general, THP-1 cells exhibit a large, round, single-cell morphology. The cells were derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia. Some of their … Meer weergeven THP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein interactions, and Meer weergeven • Cellosaurus entry for THP-1 Meer weergeven WebWe report the establishment of a subclone of the human monocytic cell line U937, termed C119/9, with unusual cytokine regulation of MHC class II expression. In contrast to the …
WebArticle Snippet: Human acute monocytic leukemia cell line THP-1 (ATCC TIB-202) was cultured in RPMI 1640 medium (HyClone, Logan, UT, USA) with 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA) at 37 °C in 5% CO 2 . Techniques: Cotransfection, Transfection, Expressing, Quantitative RT-PCR, Western Blot, Infection. Web7 nov. 2016 · Human lung adenocarcinoma (A549), cervical cancer cells (HeLa), epidermoid carcinoma (A431), breast cancer (Mcf-7), and acute monocytic leukemia (THP-1) cell lines were obtained from the American Type Culture Collection (ATCC). Cells were respectively maintained in RRMI1640 supplemented with 10% fetal bovine serum.
WebWe report the establishment of a subclone of the human monocytic cell line U937, termed C119/9, with unusual cytokine regulation of MHC class II expression. In contrast to the parental U937 cell line, only tumor necrosis factor (TNF)-alpha, and not interferon (IFN)-gamma induces the expression of MHC class II antigens on C119/9 cells, and …
WebTHP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder research, immunology …
WebThis reporter cell line is useful for monitoring the activity of cell signal transduction pathways in immune cells, specifically detecting the activation of the JNK signaling pathway are triggered by stress, inflammatory cytokines, mitogens, and oncogenes. Handling information Unpacking and storage instructions screen recording from pcWebCell line: MONO-MAC-6 : DSMZ no.: ACC 124: Species validation: human (Homo sapiens)Cell type: acute monocytic leukemia : Origin: established from the peripheral blood of a 64-year-old man with relapsed acute monocytic leukemia (AML FAB M5) in 1985 following myeloid metaplasia; carries t(9;11)(p22;q23) leading to KMT2A-MLLT3 (MLL … screen recording from windows 10Web8 aug. 2015 · Cell culture. Human monocytic THP-1 cells were maintained in culture in Roswell Park Memorial Institute medium (RPMI 1640, Invitrogen) culture medium containing 10 % of heat inactivated fetal bovine serum (Invitrogen) and supplemented with 10 mM Hepes (Gibco, #15630-056), 1 mM pyruvate (Gibco, #11360-039), 2.5 g/l D-glucose … screen recording full crackWebGenerating primary human monocyte-derived macrophages and dendritic cells may be a suitable alternative to monocytic cell lines in applications where phenotypical relevance is of critical concern, ... Liu M, Wu M. Induction of human monocyte cell line U937 differentiation and CSF-1 production by phorbol ester. Exp Hema 20(8):974-979, 1992. 6. screen recording functionalityWebMonocytic Leukemia Cell Line Infection of a monocytic leukemia cell line in vitro, however, induces a Th2-type response in both the cell lines and in peripheral blood mononuclear cells ex vivo. From: Zika and Other Neglected and Emerging Flaviviruses, 2024 View all Topics Add to Mendeley Download as PDF About this page screen recording from browserWeb27 apr. 2010 · It was subsequently shown using human epithelial and monocytic cell lines [86,87,88] that Shiga toxin-mediated modification of the ribosome triggered not only the … screen recording gameplayWebArticle Snippet: The human monocytic leukemia cell line THP-1 (ATCC, Manassas, USA) was expanded in 75 cm 2 culture flasks (Greiner Bio-One, Kremsmünster, Austria) in RPMI 1640 (Gibco, Carlsbad, USA) supplemented with 10% heat-inactivated fetal calf serum (FCS) (Gibco) and 1% penicillin-streptomycin (Gibco) in an incubator (37°C, 5% CO 2 , … screen recording function